Detecting Chlamydia trachomatis by direct immunofluorescence using a Cytobrush sampling technique.

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Detecting Chlamydia trachomatis by direct immunofluorescence using a Cytobrush sampling technique.

We compared two different methods of collecting endocervical samples for examination by direct immunofluorescence for Chlamydia trachomatis. A cervical Cytobrush gave better results than a dacron swab. Further studies should be performed to assess the value of alternative sampling methods to detect this organism.

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Cytobrush sampling technique

Chlamydiae are now commonly detected in genital tract specimens in routine diagnosis by direct immunofluorescence. Direct immunofluorescence is as sensitive and specific as conventional culture.' Studies in our laboratory also suggest that direct immunofluorescence may be a valuable test for cure.2 One of the problems encountered with direct immunofluorescence, as with culture, is the need for ...

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Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence.

This study compared the direct visualisation of elementary bodies in urogenital smears by the direct immunofluorescence test with the isolation of Chlamydia trachomatis in McCoy cell cultures treated with cytochalasin B. C trachomatis was isolated from 41 (16.4%) of 250 unselected specimens in McCoy cell cultures treated with cytochalasin B. Of the 41 culture positive specimens 37 (90%) were di...

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A comparison of cytobrush and cotton swab sampling for the detection of Chlamydia trachomatis by cell culture.

A cytobrush was compared with a cotton-tipped aluminium shafted swab for the collection of 2024 paired endocervical specimens for the culture of Chlamydia trachomatis. There was no significant advantage with the use of either device with respect to the number of positive specimens detected or the number of inclusions present in positive specimens. However, the use of cytobrushes resulted in an ...

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ژورنال

عنوان ژورنال: Sexually Transmitted Infections

سال: 1988

ISSN: 1368-4973

DOI: 10.1136/sti.64.4.245